基因重组质粒的构建与抽提

摘要....................................................................................................................................................Ⅰabstract............................................................................................................................................Ⅱ引言.......................................................................................................................................................11材料与仪器、用品.........................................................................................................................22原理与方法......................................................................................................................................3

2.1获取目的基因.......................................................................................................................3

2.1.1提取rna.................................................................................................................32.1.2rna反转录为cdna............................................................................................32.1.3聚合酶链式反应......................................................................................................42.2回收目的基因.......................................................................................................................5